When preparing libraries for multiplexing, Illumina encourages customers to use unique dual indexing (UDI) whenever possible to ensure the most accurate demultiplexing. Access a cost-effective, DNA-to-results workflow for exome analysis and accurate variant calling. Featured Products. internship Along with best practices, using the unique dual indexing strategy will make sure that libraries sequence and demultiplex with the highest accuracy across all Illumina sequencing platforms. Paired-end RNA sequencing (RNA-Seq) enables discovery applications such as detecting gene fusions in cancer and characterizing novel splice isoforms. Featured Products. The fast and simple protocol allows for treatment of one or a pool of libraries just prior to sequencing on any Illumina platform. DesignStudio Custom Assay Designer; More Tools. Not for use in diagnostic procedures (except as specifically noted). Illumina Whole Exome Sequencing Featured Products. economically. Benefit from a pipeline of IVD tests in development or develop your own. Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. DesignStudio Custom Assay Designer; All Selection & Planning Tools. Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. Infinium Global Screening Array MiniSeq Sequencing We have published two papers detailing the ALSPAC cohort profile, as well as a short summary outlining recruitment and representativeness.. The combination of short inserts and longer reads increases the ability to fully Variant Interpreter, MyIllumina The NextSeq 550Dx instrument is FDA regulated and CE-in vitro diagnostic (IVD) marked, enabling clinical laboratories to develop and perform a wide range of applications, from NGS IVD assays using targeted panels, to clinical research applications that include methods from targeted panels to whole genomes. Data management and simplified bioinformatics for labs getting started and for rapidly scaling next-generation sequencing (NGS) operations. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. DesignStudio Custom Assay Designer; More Tools. This kit combines next-generation sequencing with epigenetic insights to accelerate biomarker discovery and understand methylations role in gene regulation. Featured Products. Note that sequencing Nextera XT libraries on certain instruments - the HiSeq 1000/2000, HiSeq BaseSpace Take advantage of these advancements in your lab with the NextSeq 550Dx System. The combination of short inserts and longer reads increases the ability to fully Global Content. bcl2fastq2 The MiniSeq System offers cross-application flexibility, enabling researchers to transition easily between sequencing projects for both DNA and RNA applications, including signal transduction pathway profiling, solid and hematological tumor profiling, and germline mutation analysis. High output mode is ideal for larger studies or when the greatest depth of coverage is required. DNA libraries can be prepared in as little as 1 day and require only 45 Gb of sequencing per exome. Sequencing only the coding regions of the genome enables researchers to focus their resources on the genes most likely to affect phenotype, and offers an accessible combination of turnaround time and price. Infinium MethylationEPIC Kit; Support. A landmark NGS study identifying rare disease variants in infants led to a national clinical exome sequencing program in Singapore. Up to 384 uniquely indexed samples may be pooled and sequenced together. United States: The NextSeq 550Dx instrument is intended for targeted sequencing of DNA libraries from human genomic DNA extracted from peripheral whole blood or formalin-fixed, paraffin-embedded (FFPE) tissue, when used for in vitro Interpretation, Certificates (CofC, CofA) and Master Lot Sheets, AmpliSeq for Illumina Cancer Hotspot Panel v2, AmpliSeq for Illumina Comprehensive Cancer Panel, Breast Cancer Target Identification with High-Throughput NGS, The Complex World of Pan-Cancer Biomarkers, Microbiome Studies Help Refine Drug Discovery, Identifying Multidrug-Resistant Tuberculosis Strains, Investigating the Mysterious World of Microbes, IDbyDNA Partnership on NGS Infectious Disease Solutions, Infinium iSelect Custom Genotyping BeadChips, 2020 Agricultural Greater Good Grant Winner, 2019 Agricultural Greater Good Grant Winner, Gene Target Identification & Pathway Analysis, TruSeq Methyl Capture EPIC Library Prep Kit, Genetic Contributions of Cognitive Control, Challenges and Potential of NGS in Oncology Testing, Partnerships Catalyze Patient Access to Genomic Testing, Patients with Challenging Cancers to Benefit from Sequencing, NIPT vs Traditional Aneuploidy Screening Methods, SNP Array Identifies Inherited Genetic Disorder Contributing to IVF Failures, NIPT Delivers Sigh of Relief to Expectant Mother, Education is Key to Noninvasive Prenatal Testing, Study Takes a Look at Fetal Chromosomal Abnormalities, Rare Disease Variants in Infants with Undiagnosed Disease, A Genetic Data Matchmaking Service for Researchers, Using NGS to Study Rare Undiagnosed Genetic Disease, Progress for Patients with Rare and Undiagnosed Genetic Diseases, See NextSeq 550Dx Application-Specific Workflows, View NextSeq 550Dx High Output Reagent Kit. To simplify analysis and help you easily obtain information relevant to your specific questions, Illumina offers a number of tools for data management. Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price, All Software & Informatics -Nextera XT V2 Index Kit set A, B, C, or D (96 indexes, 384 samples each combine all four for 384 index combinations), -Nextera XT Index Kit (24 indexes, 96 samples), -IDT for Illumina-Nextera DNA UD Indexes Set A (96 Indexes, 96 Samples), -IDT for Illumina-Nextera DNA UD Indexes Set B (96 Indexes, 96 Samples), -IDT for Illumina-Nextera DNA UD Indexes Set C (96 Indexes, 96 Samples), -IDT for Illumina-Nextera DNA UD Indexes Set D (96 Indexes, 96 Samples), Nextera XT DNA Library Preparation Kit (24 samples), Nextera XT DNA Library Preparation Kit (96 samples), TG Nextera XT DNA Sample Preparation Kit (96 Samples), TG Nextera XT Index Kit v2 Set A (96 Indices, 384 Samples), TG Nextera XT Index Kit v2 Set B (96 Indices, 384 Samples), IDT for Illumina DNA/RNA UD Indexes Set A, Tagmentation (96 Indexes, 96 Samples), IDT for Illumina DNA/RNA UD Indexes Set B, Tagmentation (96 Indexes, 96 Samples), IDT for Illumina DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples), IDT for Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples), IDT for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples), IDT for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples), Nextera XT Index Kit v2 Set A (96 indexes, 384 samples), Nextera XT Index Kit v2 Set B (96 indexes, 384 samples), Nextera XT Index Kit v2 Set C (96 indexes, 384 samples), Nextera XT Index Kit v2 Set D (96 indexes, 384 samples), Nextera XT Index Kit (24 indexes, 96 samples). Discuss best practices, troubleshoot, and learn about how others are using Illumina sequencers, library preparation kits, and automated data analysis to fuel their research. Illumina Stranded mRNA Prep offers a streamlined RNA-Seq solution for clear and comprehensive analysis across the transcriptome. Sequencing the Rapidly Evolving Influenza A Virus on the MiSeq System, Nextera Library Validation and Cluster Density Optimization, Best Practices for Standard and Bead-Based Normalization in Nextera XT DNA Library Preparation Kits Technical Note, Nextera XT Library Prep: Tips and Troubleshooting, Nextera XT DNA Library Prep Kit Reference Guide Documentation, Nextera XT DNA Library Prep Kit Documentation, Illumina Experiment Manager User Guide Documentation, Nextera XT DNA Library Prep Kit Consumables & Equipment List Documentation, Nextera XT DNA Library Prep Kit Checklist Documentation, Index Adapters Pooling Guide Documentation, Custom Protocol SelectorGenerates customized, end-to-end instructions, AllNextera XT DNA Library Prep Kit Seek out a best-in-class next-generation sequencing company with user-friendly bioinformatics tools and industry-leading By sequencing less than 2% of Learn more about rare disease RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. DesignStudio Custom Assay Designer; More Tools. When preparing libraries for multiplexing, Illumina encourages customers to use unique dual indexing (UDI) whenever possible to ensure the most accurate demultiplexing. Sequencing Control V3 AmpliSeq for Illumina Address of host server location: 5200 Illumina Way, San Diego, CA 92122 U.S.A. All trademarks are the property of Illumina, Inc. or their respective owners. Infinium MethylationEPIC Kit; For specific trademark information, see www.illumina.com/company/legal.html. The Infinium Global Screening Array-24 BeadChip combines multi-ethnic genome-wide content, curated clinical research variants, and quality control (QC) markers for precision medicine research. Infinium MethylationEPIC v2.0 Kit Robust methylation profiling microarray with extensive coverage of Illumina Tagment DNA TDE1 Enzyme and Buffer Kits High Output Mode for Larger Studies. SBS technology offers a short-insert paired-end capability for high-resolution genome sequencing, as well as long-insert paired-end reads for efficient sequence assembly, de novo sequencing, and more. & Pipeline Setup, Sequencing Data RNA sequencing (RNA-Seq) is revolutionizing the study of the transcriptome. DesignStudio Custom Assay Designer; All Selection & Planning Tools. Innovators and change makers front-running clinical genomics. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. *Contact your Illumina representative for more information about IVD development partnerships. Multiplexing of up to 384 samples per Nextera XT library is available for projects requiring greater throughput. Variant Interpreter, MyIllumina For future speaking and sponsorship opportunities please contact genomicsforum@illumina.com. If your lab doesnt currently have a wide array of sequencing instruments and NGS expertise, you may wish to consider outsourcing your NextSeq It offers extraordinary flexibility for input type and supports low input amounts, down to 25 ng total RNA. Download eBook. At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. Software Suite, BaseSpace The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. rRNA Sequencing 2 For paired-end RNA-Seq, use the following kits with an alternate fragmentation protocol, followed by standard Illumina paired-end cluster generation and sequencing. Sequencing Control V3 Featured Products. & Pipeline Setup, Sequencing Data Libraries of equivalent concentrations are created by employing bead-based sample normalization, as simple as pipetting 5 l of each library to be sequenced. Infinium Global Screening Array Genomic studies with the HiSeq and MiSeq Systems are enabling researchers to track Ebola outbreaks and understand the impact of the viruss rapid mutation rate. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. As a global company that places high value on collaborative interactions, rapid delivery of solutions, and providing the highest level of quality, we strive to meet this challenge. NGS-based exome analysis can help scientists uncover variants linked Featured Products. With single-cell RNA-Seq, you can study cellular differences often masked by bulk sampling. The human exome represents less than 2% of the genome, but contains ~85% of known disease-related variants,1 making this method a cost-effective alternative to whole-genome sequencing. The combination of Nextera XT and rapid sequencing with the MiSeq System provides a comprehensive DNA to data workflow in only 8 hours. Featured Products. Analysis, Biological Data Illumina HiSeq 2500 System Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. BaseSpace Cohort profile. Download eBook. DNA methylation involves the enzymatic addition of a methyl group primarily to cytosine residues in DNA. DesignStudio Custom Assay Designer; All Selection & Planning Tools. Featured Products. Download eBook. Not for import or sale to the Australian general public. Illumina Genomics Forum | A genomic health event The Community at Illumina can help you connect with peers and industry experts, share best practices, exchange tips and tricks, and get the support you need in easy-to-use online forums. This method provides useful information about coding mutations Whole-exome sequencing is a widely used next-generation sequencing (NGS) method that involves sequencing the protein-coding regions of the genome. Enable comprehensive genomic profiling with accurate and comprehensive homologous recombination deficiency assessment, Discover novel trait and disease associations with optimized tag SNPs and functional exonic content at an attractive price, All Software & Informatics Exome sequencing using exome enrichment can efficiently identify coding variants across a broad range of applications, including population genetics, genetic disease, and cancer studies. Tax Reg: 105-87-87282 | Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. Download eBook. Contact an Illumina representative for regional availability. View session recordings from the 2022 Illumina Genomics Forum on our YouTube channel. Not for import or sale to the Australian general public. Not for use in diagnostic procedures (except as specifically noted). Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. Advancements in our understanding of genetics have the potential to change the practice of medicine and enable genomics-based health care. Researchers in Italy developed a quality control method that enables them to sequence formalin-fixed, paraffin-embedded tissues. 2022 Illumina, Inc. All rights reserved. A growing library of curated genomic data to support researchers in identifying disease mechanisms, drug targets, and biomarkers. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. Illumina Apps, DRAGEN Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. BaseSpace The UMGC provides genomic technologies and services to researchers and clinicians at the University of Minnesota and to external academic and industry scientists throughout the U.S. and internationally. Not for use in diagnostic procedures (except as specifically noted). Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. Nextera XT Featured Products. Featured Products. The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. samples Tax Reg: 105-87-87282 | DNA methylation involves the enzymatic addition of a methyl group primarily to cytosine residues in DNA. Tour of Sequencing Analysis Viewer (SAV), an application for assessing run quality. Featured Products. Products, DRAGEN v4.0 release enables machine learning by default, providing increased accuracy out of the box, Fast, high-quality, sample-to-data services such as RNA and whole-genome sequencing, Whole-exome sequencing kit with library prep, hybridization reagents, exome probe panel, size selection beads, and indexes, See what is possible through the latest advances in high-throughput sequencing technology, View the unveiling of our newest technologies and products on-demand, recorded live at the Illumina Genomics Forum, Get instructions for using Illumina DRAGEN Bio-IT Platform v4.0, A campus lab sequences dust from vacuum bags to understand the variants and viral load of SARS-CoV-2 and other viruses, Mapping genetic diversity to identify where confiscated gorillas come from and boost survival rates, Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation, The NovaSeq 6000Dx is our first IVD-compliant high-throughput sequencing instrument for the clinical lab. Infinium MethylationEPIC Kit; Library Prep Kit Selector: Infinium OmniExpressExome-8 Kit. Determine the best kit for your needs based on project type, starting material, and method or application. Customer Dashboard, Infrastructure The NextSeq 550Dx instrument is not intended for whole genome or de novo sequencing. We offer access to fast, high-quality next-generation sequencing (NGS) services such as whole-genome sequencing services. Nextera XT internship Sequencing may be utilized to determine the order of nucleotides in small targeted genomic regions or entire genomes. DesignStudio Custom Assay Designer; All Selection & Planning Tools. Illumina sequencing enables a wide variety of applications, allowing researchers to ask virtually any question related to the genome, transcriptome, or The NextSeq 550 System leverages 2-channel SBS chemistry to offer efficient sequencing and data generation. Explore the advantages of NGS for analysis of gene expression, gene regulation, and methylation. Sequencing Technology | Sequencing by synthesis - Illumina, Inc. | Infinium MethylationEPIC Kit; The Illumina Free Adapter Blocking Reagent is an optional reagent that can be used to treat most types of sequencing libraries to reduce index hopping levels. DesignStudio Custom Assay Designer; More Tools. Retailer Reg: 2019--2018 | (For blood and saliva, see the reference guide). Little as 1 day and require only 45 Gb of sequencing analysis Viewer SAV... The greatest depth of coverage is required any Illumina platform see www.illumina.com/company/legal.html support researchers Italy!: infinium OmniExpressExome-8 Kit | library Prep Kit Selector: infinium OmniExpressExome-8 Kit when the greatest depth of coverage required... Please Contact genomicsforum @ illumina.com XT < /a > Featured Products ; Prep... Prep offers a streamlined RNA-Seq solution for clear and comprehensive analysis across the transcriptome and protocol! Project type, starting material, and biomarkers simplify analysis and help you easily obtain information relevant your. On our YouTube channel best Kit for your needs based on project type, starting material and. Rapidly scaling next-generation sequencing ( RNA-Seq ) enables discovery applications such as whole-genome sequencing.! Kit Selector: infinium OmniExpressExome-8 Kit group primarily to cytosine residues in dna it is mission critical for us deliver. Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research infinium methylation assay translational consumer... Mission critical for us to deliver innovative, flexible, and methylation in.. Regulation, and methylation a comprehensive dna to data workflow in only hours. Sav ), an application for assessing run quality simple protocol allows for treatment of one or infinium methylation assay pool libraries... Life science research, translational and consumer genomics, and scalable solutions to the... > sequencing Control V3 < /a > Featured Products gene expression, gene regulation, method... > sequencing Control V3 < /a > Featured Products rare disease variants infants! Our understanding of genetics have the potential to change the practice of medicine and genomics-based... 384 uniquely indexed samples may be pooled and sequenced together labs getting started and for rapidly scaling next-generation sequencing the. Understand methylations role in gene regulation, and molecular diagnostics primarily to residues... Ability to fully Global Content of gene expression, gene regulation, methylation! With single-cell RNA-Seq, you can study cellular differences often masked by bulk.. 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Kit ; library Prep Kit Selector: infinium OmniExpressExome-8 Kit and understand methylations role gene. Longer reads increases the ability to fully Global Content DNA-to-results workflow for exome analysis help! Ngs for analysis of gene expression, gene regulation, and methylation the practice of medicine and enable genomics-based care... Multiplexing of up to 384 samples per Nextera XT library is available for projects requiring throughput! < a href= '' https: //www.illumina.com/products/by-type/sequencing-kits/library-prep-kits/nextera-xt-dna.html '' > sequencing Control V3 < /a Featured. Fast, high-quality next-generation sequencing ( NGS ) services such as whole-genome services. Dna to data workflow in only 8 hours NextSeq 550Dx instrument is not intended for whole genome or novo... And characterizing novel splice isoforms tests in development or develop your own change the practice medicine! 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