Streptomycin resistant and sensitive hybrids of Nicotiana tabacum + Nicotiana knightiana: Correlation of resistance with N. tabacum plastids. Somatic hybrid plants of Nicotiana glauca and Nicotiana tabacum obtained by protoplast fusion. Akad. Sci., 9, 629 (1969). Part of Springer Nature. 1980. Ohio State Univ. Fowke, L.C., Bech-Hansen, C.W., Gamborg, 0.L. Evans, D.A., Wetter, L.R., and Gamborg, O.L. Bethesda, MD 20894, Web Policies Plant Protoplast Fusion | Springer for Research & Development (1985c) Analytical and Preparative Electrofusion of Plant Protoplasts, in Oxford Surveys of Plant Molecular and Cell Biology (Miflin, B.J., ed.). Somatic hybridization between male sterile Nicotiana tabacum and N. glutinosa through protoplast fusion. Eriksson, T., and Jonasson, K., Planta, 89, 85 (1969). B. 1982. Get the most important science stories of the day, free in your inbox. The term protoplast was first introduced by Hanstein in 1880 to designate the living matter enclosed by plant cell membrane. Hahlbrock, K. Regulation of the enzymes of phenylpropanoid metabolism in relation to specific growth stages of plant cell suspension cultures. Chen, K., Wildman, S.G., and Smith, H.H. Protoplast Fusion and Citrus Improvement - Plant Breeding Reviews Z. Pflanzenzuchtg. Constabel, F., Kurz, W.G.W., Chatson, B. and Kirkpatrick, J.W. Power, J.B., Frearson, E.M., Hayward, C., George, D., Evans, P.K., Berry, S.F., and Cocking, E.C. 10, 614617 (1976). CAS PubMedGoogle Scholar, Evans, D. Agricultural Applications of Plant Protoplast Fusion. Theoret. FOIA Plant Sci. Genet. Plant Protoplast Fusion by Electroporation Electroporation Protocol: Plant Protoplast Fusion may be performed by Electroporation . Kartha, K.K., Gamborg, 0.L., Shyluk, J.P. and Constabel, F. Morphogenetic investigations on in vitro leaf culture of tomato (Lycopersicon escultentum Mill. Cell division and plant development from protoplasts of carrot cell suspension cultures. and Constabel, F. Morphogenesis and plant regeneration from callus of immature embryos of sorghum. The removal of the cell wall of one cell of each type of plant using cellulase enzyme to produce a somatic cell called a protoplast The cells are then fused using electric shock (electrofusion) or chemical treatment to join the cells and fuse together the nuclei. Plant Tissue and Cell Culture, A. Fujiwara (ed). Uptake of bacteria by isolated higher plant protoplasts. Isoenzyme patterns in soybean-Nicotiana somatic hybrid cell lines. Gen. Genet. Molec. Download Download PDF. Gosch, G. and Reinert, J. Protoplasts have been critical for studying many aspects of plant biology, including hybridization, light/chloroplast-related activities and plant defense mechanisms . Simple protoplast isolation system for gene expression - Plant Methods 69, 22922294. (1983) Culture and selection of somatic hybrids, using an auxotrophic cell line. Planta tatula L. I. Open Access PDF Protoplast Fusion Technology and Its Biotechnological Applications - AIDIC Medgyesy, P., Menczel, L., and Maliga, P. 1980. Tokumasu, S. The increase of seed fertility of Brassicoraphanus through cytological irregularity. Ultrastructural studies of the aggregation and fusion of plant protoplasts. Isolation, culture and induction of embryogenesis in protoplasts from cell suspensions of Atropa belladonna. Plant Sci. Hber, R., Physical Chemistry of Cells and Tissues, 289 (Churchill, London, 1950). 1979. Uptake of the nitrogen fixing blue-green algae Gloeocapsa into protoplasts of tobacco and maize. Lazar, G.B., Dudits, D., and Sung, Z.R. 1981. C chua lai khoai ty (tn ting Anh l pomato) (IPA: /pmt/, ting Vit: p-ma-tu) l mt loi cy nhn to (do ngi to ra) bng cch lai ghp khoai ty (potato) vi c chua (tomato). + Datura stramonium L. var. 18, 491507 (1975). Figure 7 KoNAC-GFP fusion vectors were used to determine the subcellular localization of several KoNACs in Arabidopsis thaliana protoplast cells. Z. Pflanzenphysiol 77, 292301 (1975). This is a preview of subscription content, access via your institution. 1983. Protoplast fusion was carried out between the Helminthosporium gramineum subsp. Vasil, I.K. J. Bot. Protoplasts can fully regenerate into functional plants when given proper plant growth regulators and growth medium. Naturwiss. (USA) 70, 16931696 (1973). Published 1 May 1983 Biology Bio/Technology Plant protoplasts can be fused and the fusion products cultured to produce somatic hybrid plants. Newsl. Sci. Springer, Boston, MA. Maliga, P., Lazar, G., Joo, F., Nagy, A.H., and Menczel, L. 1977. Suspensions of plant cells in microgravity. https://doi.org/10.1385/0-89603-127-6:481, Shipping restrictions may apply, check to see if you are impacted, Tax calculation will be finalised during checkout. (1970). (This new electroporation method has been developed and patented by Dr. Hagio of the Japanese Institute of Agrobiological Resources). Google Scholar. Sign up for the Nature Briefing newsletter what matters in science, free to your inbox daily. Proc. (1974) Plant protoplast fusion and growth of intergeneric hybrid cells. and Vasil, I.K. J. Genet. Nonchromosomal inheritance in higher plants as studied by somatic cell hybridization. Stomatal analysis of citrus somatic hybrids 297 Notas Cientficas Stomatal analysis of citrus somatic hybrids obtained by protoplast fusion Maria Anglica Pereira de Carvalho Costa(1), Weliton Antonio Bastos de Almeida(1), Francisco de Assis Alves Mouro Filho(2), Beatriz Madalena Januzzi Mendes(3) and Adriana Pinheiro Martinelli Rodriguez(3) (1)Escola de Agronomia da Universidade Federal . 1976. and of Datura innoxia Mill. Scowcroft, W. F. and Adamson, J.A. Carlsberg Res. Plant Breeding Reviews, Volume 8. Somatic hybrids. J. Genet. Theoret. Planta 120, 215227 (1974). What is protoplast fusion in plant tissue culture? Nature (London) 239, 455456 (1972). Genetic Engineering for Nitrogen Fixation pp 299316Cite as, Part of the Basic Life Sciences book series (BLSC,volume 9), For some time we have been exposed to popular articles describing the magic and more recently some of the potential terror which lies before us as scientists acquire sophisticated skills in the engineering of new genes. Bot. 139: 14. Plant protoplast fusion and hybridization Basic Life Sci. Genet. Most interesting is the fusion between human cells and carrot protoplasts. and Keller, W.A. (1977). Yu. Reproducible protocols for protoplast culture and regeneration to plants have been established for a large number of crop species within this family, such as oilseed rape, turnip rape, cabbage, and Indian mustard (1). (Eds.) Proc. Institutions (1) 01 Apr 1981-Biochemical Society Transactions (Portland Press Ltd.)-Vol. Somatic Cell Gen. (1976). Appl. Open Access articles citing this article. To obtain Ruesink, A. W., and Thimann, K. V., Science, 154, 280 (1966). and Wetter, L.R. Epub 2014 Jan 3. (1977). A method for the isolation of plant protoplasts and vacuoles. 63: 193198. Get the most important science stories of the day, free in your inbox. Steward, F. C., Plant Physiology, 8 (Academic Press, 1969). Cytogenetics of the Genus Nicotiana. Sofia, Bulgaria: State Printing House. Carrot protoplast can be fused by 28% PEG 1500 and fusion can be promoted by ca++ ion at concentration of 3.5 mM. Plant Physiol. Genetic transformation in plants. Google Scholar. Selection and characterization. Annu. 301327 (1974). Dokl. and King, J.M. Menczel, L., Lazar, G., and Maliga, P. 1978. Zelcer, A., Aviv, D., and Galun, E. 1978. Some features of somatic hybrids between Solarium tuberosum and S. chacoense and its F1 sexual progeny. Protoplast fusion and plant regeneration in tobacco. Steward, F.C. Dudits, D., Hadlaczky, G.Y., Bajszar, G.Y., Koncz, C.S., and Lazar, G. 1979. Protoplasts may also be used for plant breeding, using a technique called protoplast fusion. The isolation of heterokaryons and hybrids by a selective system using irreversible biochemical inhibitors. Molec. Spontaneous Fusion 2. Butenko, R., Kuchko, A., and Komarnitsky, I. How do spheroplasts and protoplasts differ? - igris.dailyview.tw Article CrossRef Recently, the fusion of plant protoplast and animal protoplast has been reported. Iwai, S., Nagao, T., Nakata, K., Kawashima, N., and Matsuyama, S. 1980. Nat Biotechnol 1, 253261 (1983). Cocking, E.C. and Spurgeon, D. Triticale. J. Vardi, Aliza, Spiegel-Roy, P. and Galun, E. Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. To detect the effect of gene mutation on protein localization, OsSPL26 F98Y-GFP fusion proteins were expressed in rice protoplasts. Microdroplet culture: Tobacco plants from single mesophyll protoplasts. Symposium on Experimental Mutagenesis in Plants, October 1976, Varna, Bulgaria. Enzymolysis Appl. Science 173: 6769. Plant Tissue Culture, Tokyo, Japan. Somatic hybridization by fusion of protoplasts. 1990 Dec;3(3):168-72. Gen. Genet. 16, 465470 (1976). 66, 127130. 60: 197214. I. Genet. and Cocking, E.C. Macmillan, New York (in press). II. Very recently the following appeared in a well known newspaper: The first aim of scientists is to introduce into cereals the ability to fix nitrogen which would massively increase yield and eliminate the need for nitrogen fertilizers.. Plant Sci. Spontaneous fusion: Cell fusion is a process integral to plant development. Child, J.J. New developments in nitrogen fixation research. Genet. Gregory, D. W., and Cocking, E. C., J. Carlson, P.S., Smith, H.H., and Dearing, R.D. In document Plant Tissue Culture.pdf (Page 187-191) Freshly isolated protoplasts will fuse if brought into intimate contact and held together for a few minutes. Single gene mutations in tomato plants regenerated from tissue culture. IU. Genetic analysis of somatic hybrid plants. Wullems, G.J., Molendij, K.L., and Schilperoort, R.A. 1980. Expression of cycloheximide resistance in carrot somatic hybrids and their segregants. Genetic evidence for the hybrid nature of somatic hybrids from Datura innoxia Mill. This area is exciting because protoplasts from different origins can be fused together to form new genome combinations The isolated protoplast is unusual as the outer plasma membrane is totally exposed and is the only barrier between the external environment and the interior of living cell. Nauk USSR. and Hasenkampf, C.A. Biotechnology In Agriculture And Forestry Vol 9 Plant Protoplasts And Protoplast Isolation- mechanical and enzymatic methods Crop Sci. A history of plant biotechnology: from the Cell Theory of Schleiden and Schwann to biotech crops. 2. biotechnology-in-agriculture-and-forestry-vol-9-plant-protoplasts-and-genetic-engineering-ii 2/12 Downloaded from stats.ijm.org on November 20, 2022 by guest between Nicotiana glauca and Nicotiana 162: 113119. Genet. Google Scholar. Larkin, P.J. Protoplast-based transient expression assays are very convenient as they allow rapid and high-throughput analysis of gene expression, protein subcellular localization, protein activity and protein-protein interactions [ 10 ]. Article PROTOPLASMIC FUSION - YouTube The use of isoenzymes in distinguishing the sexual and somatic hybrids in callus cultures derived from Nicotiana. Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Deutsch. 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